Objective This research analyzed the latent profile of occupation fatigue of nurses and discussed the influencing factors of occupation fatigue among different types. Methods Based on the Effort-Reward Imbalance Model,we used the convenience sampling method to select 551 nurses from 2 tertiary hospitals in Shanghai in December 2020. The demographic characteristics,Occupation Fatigue Exhaustion Recovery Scale and Effort-Reward Imbalance Questionnaire were used to make a cross-section survey of nurses’ occupation fatigue,and the latent profile analysis was carried out with the results of 3 explicit indexes of Occupational Fatigue Exhaustion Recovery Scale. At the same time,the influencing factors of occupational fatigue of nurses were explored by univariate analysis and ordered multi-classification logistic regression. Results The 55l nurses could be divided into 3 latent subgroups,including low fatigue and high recovery（14.5%）,medium fatigue and medium recovery（54.3%） and high fatigue and low recovery（31.2%）. The influencing factors of nurses’ occupational fatigue included total weekly working hours,work-family conflict,external effort,over commitment and reward（P<0.05）. Conclusion The occupational fatigue of nurses can be divided into 3 subgroups. The nursing managers can make individualized psychological interventions according to the influencing factors to improve the self-regulation ability of nurses,so as to reduce the degree of fatigue.